Analytical Techniques-Purification of Biomolecules
This course covers various types of chromatography, gel filtration and other analytical techniques in the purification of biomolecules, especially proteins.
The various techniques and analysis involved in the complex purification of biomolecules is covered in this free online course. Learn the definition of gel filtration chromatography and affinity chromatography, which works on the principle of mutual recognition forces between a ligand and a receptor, will also be discussed.
What You Will Learn In This Free Course
Chromatography
In this module, Chromatography, you will learn about Chromatography, a technique that allows scientists to separate molecules of interest from a crude mixture. You will also learn about different Chromatography Techniques and how to use Iron-Exchange Chromatography.
Diploma in Analytical Techniques in Purification of Biomolecules – First Assessment
You must score 80% or more to pass this assessment.; Module
Gel Filtration Chromatography
In this module, Gel Filtration Chromatography, you will learn about the different safety precautions to take when performing the gel filtration chromatography. You will also learn how to conduct Column Packing Experiments and validation procedures.
Affinity Chromatography
In this module, Affinity Chromatography, you will learn about the Operation of Affinity Chromatography and the steps in the process of generating antibodies. You will also learn about Bio-affinity Chromatography and the Metal-affinity Chromatography Protein Purification process.
Diploma in Analytical Techniques in Purification of Biomolecules – Second Assessment
You must score 80% or more to pass this assessment.; Module
Course assessment
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