Experimental Biotechnology

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In the current MOOCs course I have put effort to briefly discuss different analytical techniques and their potential in solving the scientific problems. We are taking several scientific problems or questions which can be solved by using these techniques. 

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COURSE LAYOUT

Week 1: Buffering solution in Biological System:Good Lab Practices, Concept of buffering and Preparation of Solutions and Reagents
Week 2: Electrophoresis (Part 1):Basic Concept of Electrophoresis, performance of electrophoresis and its applications
Week 3: Electrophoresis:Horizontal Gel electrophoresis, discussion about scientific questions and related experiments to solve them utilizing electrophoresis
Week 4: Chromatography (Part I): Analytical techniques in purification of biomolecules; Column chromatography, HPLC.
Week 5: Chromatography (Part II) : Analytical techniques in purification of biomolecules; Column chromatography, HPLC.
Week 6: Chromatography (Part III) : Discussion about scientific questions and related experiments to solve them utilizing chromatography
Week 7: Immunology Techniques: Immunological tools, Antibody Generation and Purification, ELISA, Radial Immuno-diffusion, Western blotting and Immuno-precipitation.
Week 8: Cell Biology Techniques (Part 1): Basics of cell-culture, isolation of cells from tissue and fractionation.
Week 9: Cell Biology Techniques (Part 2): Immuno-localization, cell sorting, and discussion about scientific questions and related experiments to solve them utilizing cell biology techniques.
Week 10:Molecular Biology (Part 1):Primer designing, Polymerase chain reaction, Different variants of PCR and their applications.
Week 11: Molecular Biology (Part 2) Blotting Techniques, DNA and protein sequencing techniques and their applications
Week 12:Summary and Conclusions

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    Experimental Biotechnology
    Experimental Biotechnology

    Free!

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