Microbial Physiology and metabolism
Microbial physiology and metabolism provides information on sources of energy and its utilization by microorganisms. Microorganisms play important role in environment as producers, consumers and decomposers. These are the only group of organisms which takes part in all three important stages of ecosystem. Understanding microbial physiology has greater application in industry, developing medicine and even in agriculture. Microorganisms being Lilliput in size behaves like tiny factories to produce several enzymes, metabolites of industrial importance which are regulated by specific genes under the influence of environmental factors. Regulating these two parameters will provide greater output of the metabolites of the choice. To know the specific parameters it is important to understand physiology of these organisms in detail.
COURSE LAYOUT
First Week
Day 1
Microbial enzymes: Structure and Classification
First Week
Day 2
Mechanism of Enzyme actions: Lock and key model, induced fit theory
First Week
Day 3
Factors affecting rates of enzyme mediated reactions
First Week
Day 4
The role of ATP in metabolism
First Week
Day 5
? MCQ
? Assignments
Second Week
Day 1
Definitions of growth and generation time, measurement of microbial growth and specific growth rate
Second Week
Day 2
Batch and Continuous culture
Second Week
Day 3
Phases and types of growth curve and its industrial application
Second Week
Day 4
Microbial growth in response to temperature, pH, solute and water activity, oxygen, pressure and radiation
Second Week
Day 5
? MCQ
? Assignments
Third Week
Day 1
Classification of bacteria based on nutrients
Third Week
Day 2
Membranes of microorganisms, Ion channels
Third Week
Day 3
Passive and facilitated diffusion, Primary and secondary active transport, concept of uniport, symport and antiport
Third Week
Day 4
Group translocation and Iron uptake
Third Week
Day 5
? MCQ
? Assignments
Fourth Week
Day 1
Photosynthetic pigments and apparatus in bacteria
Fourth Week
Day 2
Photophosphorylation
Fourth Week
Day 3
C3 and C4 pathways
Fourth Week
Day 4
Difference between oxygenic and anoxygenic photosynthesis
Fourth Week
Day 5
? MCQ
? Assignments
Fifth Week
Day 1
Mode of nutrition in Hydrogen and Nitrifying bacteria
Fifth Week
Day 2
Mode of nutrition in Purple sulfur bacteria, Non-sulfur bacteria and Green sulfur bacteria
Fifth Week
Day 3
Mode of nutrition in methylotrophs and methanogens
Fifth Week
Day 4
Utilization of light energy by halobacteria
Fifth Week
Day 5
? MCQ
? Assignments
Sixth Week
Day 1
Concept of aerobic respiration, anaerobic respiration and fermentation
Sixth Week
Day 2
Sugar degradation pathways i.e., EMP, ED and Pentose phosphate pathway
Sixth Week
Day 3
TCA cycle and Electron transport chain
Sixth Week
Day 4
Comparison of mitochondrial and bacterial ETC, electron transport phosphorylation, uncouplers and inhibitors
Sixth Week
Day 5
? MCQ
? Assignments
Seventh Week
Day 1
Fates of pyruvate, Pasteur effect and industrial importance of fermentation
Seventh Week
Day 2
Alcohol fermentation
Seventh Week
Day 3
Lactate fermentation (homo fermentative and hetero fermentative pathways)
Seventh Week
Day 4
Concept of linear and branched fermentation pathways
Seventh Week
Day 5
? Quizzes
? Assignments
? Paragraph and essay questions
Eighth Week
Day 1
Utilization of Lactose and Galactose
Eighth Week
Day 2
Utilization of Maltose and Mannitol
Eighth Week
Day 3
Degradation of cellulose, starch and glycogen
Eighth Week
Day 4
Conversion of biomass to energy using microorganisms
Eighth Week
Day 5
? MCQ
? Assignments
Ninth Week
Day 1
Mechanism of nitrogen fixation
Ninth Week
Day 2
Symbiotic and non symbiotic nitrogen fixation
Ninth Week
Day 3
Biosynthesis of amino acids
Ninth Week
Day 4
Degradation amino acids
Ninth Week
Day 5
? MCQ
? Assignments
Tenth Week
Day 1
Oxidative stress
Tenth Week
Day 2
Thermal stress
Tenth Week
Day 3
Starvation stress and stringent response
Tenth Week
Day 4
Aerobic to anaerobic transitions
Tenth Week
Day 5
? MCQ
? Assignments
Eleventh Week
Day 1
Exam preparation and assessment of assignments
Eleventh Week
Day 2
Exam preparation and assessment of assignments
Eleventh Week
Day 3
Exam preparation and assessment of assignments
Eleventh Week
Day 4
Exam preparation and assessment of assignments
Eleventh Week
Day 5
Exam preparation and assessment of assignments
Twelfth Week
Day 1
Assessment at the end of the course, which comprises of 40% of Online or In-term assessment and 60% Proctored end term exam.
Twelfth Week
Day 2
Assessment at the end of the course, which comprises of 40% of Online or In-term assessment and 60% Proctored end term exam.
Twelfth Week
Day 3
Assessment at the end of the course, which comprises of 40% of Online or In-term assessment and 60% Proctored end term exam.
Twelfth Week
Day 4
Assessment at the end of the course, which comprises of 40% of Online or In-term assessment and 60% Proctored end term exam.
Twelfth Week
Day 5
Assessment at the end of the course, which comprises of 40% of Online or In-term assessment and 60% Proctored end term exam
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